Bacteria transformation, Mini prep and PCR analysis

Bacterial transformation is a useful tool in molecular genetics, being used to create genetically modified bacteria capable of adapting and surviving in conditions that the parent culture would not. Introducing a plasmid into a bacterial host can be a difficult procedure, requiring molecular techniques. The materials and methods provided give an overall instruction on how to prepare and proceed with mini prepping bacterial samples from pellets, carrying out a PCR on purified DNA and the bacterial transformation and gene cloning. The following explains the volumes used in this procedure along with the cells and consumables used.